How to make a hybridoma.

نویسنده

  • E. A. Lerner
چکیده

The use of monoclonal antibodies produced by the hybridoma technique has had a profound impact on the biological and medical sciences. While the general technique for the production of monoclonal antibodies is available from the numerous papers which report their use, there is a need for a source which describes the procedure and its pitfalls in detail so that an investigator who decides to make hybridomas can do so quickly and easily. This guide provides such a source.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

It Won’t Be Easy: How to Make Universal Pharmacare Work in Canada

One of the glaring gaps in Canada’s universal healthcare system is the low level of public financing of prescription drugs - 42.7% of total spending in 2018. At the federal level there is renewed interest in moving towards universal coverage, supported by a recently commissioned report on how to achieve it. It will take superb political navigation to extract Canadian ph...

متن کامل

How to Make Welayate Faqih as a Discourse in the Curricula of Islamic Republic of Iran's Educational System

Current article is aimed at introducing discourse making approach as the desired approach in transferring ma'aref of Islamic revolution, especially the theory of Welayat-e faqih. First a general description of the current status in transferring the theory of welayat-e faqih was offered. Then the desired approach, i.e. discourse making approach was introduced and conceptology of main terms in th...

متن کامل

Chromosomal Variation in Three Human-Mouse Hybridoma Cell Lines after Various Passaging Intervals as Assessed with Two Different Staining Methods

Objective(s) The main objective of this study was to investigate the status of chromosome stability in 3 human-mouse hybridoma cell lines over a period of time in various passages. Materials and Methods Metaphase spreads from 3 human-mouse cell lines (HF2X653, SPMO-4 and F3B6) that had been cultured in 4 successive passages, from 1 to 4 weeks, were prepared and analyzed. Metaphase chromosome...

متن کامل

فراوانی آسیب‌های کروموزومی ناشی از داروی بلئومایسین سولفات در سلول‌های هیبریدومای F3B6 و HF2x653 در مقایسه با سلول‌های غیرهیبرید والدی

  Background and Objective: Hybrid cells are made from fusion of two or more somatic cells. After formation chromosomes are located in one membrane . . So nucleic condition of each fused cell has changes and two genomes and chromosomes interact with each other. Locating the genes in new nucleic and cytoplasmic condition and great chromosomal rearrangement in these new formed cells can affect th...

متن کامل

How to Diagnose Arterial and Venous Ulcers and Make Good Medical Decisions

چگونگی مراقبت از یک زخم مزمن اغلب به علت آن بستگی دارد. برای مثال زخم های پا ممکن است در اثر بیماریهای شریانی و یا وریدی بوجود آیند. هر چند زخم های مزمن پا به دلایل متعددی بروز می کنند ولی اختلال در گردش خون شایعترین علت می باشد. اخذ تصمیمات مهم درمانی از قبیل درمان با استفاده از فشار و یا درمان تهاجمی جهت رفع عفونت همگی بستگی به این دارد که منشاء زخم شریانی است با وریدی .

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • The Yale Journal of Biology and Medicine

دوره 54  شماره 

صفحات  -

تاریخ انتشار 1981